POs-Ca™

POs-Ca™

  • Phosphoryl Oligosaacharides of Calcium
  • POs-Ca,a calcium salt of phosphoryl oligosaccharides made from potato starch, is a derivative of maltooligosaccharides and is characterized as a highly water-soluble calcium carrier.

  • Structure and manufacturing process of POs-Ca

  • Potato starch is a glucose po contains ester-linked phosphate groups. Phosphoryl oligosaccharides (phosphoryl maltooligosaccharides) are produced through enzymatic hydrolysis of potato starch, POs-Ca is produced as a calcium salt of phosphoryl oligosaccharides.
  • POs-Ca comprises maltooligosactaosecharides (maltotriose to maltoctaose) in which at least one or two phosphate groups are bound to a calcium ion.

  • Characteristics

  • ●High water solubility; dissolves up to 70%
  • ●Soluble in 20% or less ethanol aolution
  • ●Weak electrolyte
  • Skin Care

    1st barrier

  • Promotion of gene expression for stratum corneum conditioning and skin hydration
  • Expression levels of genes in normal human epidermal kerationocyter (NHEKs) cultured with/without 0.1% POs-Ca for 144 hours was analyzed by HPTLC.After visualizing the spots in the HPTLC plate, the amounts of ceramides and cholesterol were quantitatively determined using a densitometer (Fig.B)
  • Promotion of intercellular lipid production
  • intercellular lipid in NHEKs cultured with/without 0.1% POs-Ca for 144 hours was analyzed by HPTLC. After visualizing the spots in the HPTLC plate, the amounts of ceramides and cholesterol were quantitatively determined using a densitometer (Fig. C).
  • Promotion of cell differentiation
  • Production of involucrin protein, a keratinocyte differentiation marker, was promoted in NHEKs cultured for 144 hours in the presence of 0.1% POs-Ca compared with that in the absence of POs-Ca (Fig. C).
  • 2nd barrier

  • Enhancement of tight junction (TJ) formation
  • NHEKs were cultured with 0.1% POs-Ca to determine the following:
  • Expression levels of genes for TJ related proteins, occludin and claudin-4 in NHEKs cultured for 144 hours ( Fig. B).
  • lmmunostaining of occludin and claudin-4 in NHEKs cultured for 144 hours (Fig. B)
  • Continuous honeycomb localization of TJ proteins at cell borders was observed only in POS-Ca treated cells.
  • Percutaneous administration of POs-Ca is significan effective for inproving skin hydration,barrier function,stratum corneum condition, and skin texture.
  • Scalp Care

  • Genes related to angiogenesis (growth of new blood vessele), anti-apoptosis of DPCs, and extracellular matrix were upregulated by POs-Ca.
  • Proliferation of follicle dermal papilla cells

  • PDCs were plated on a 96-well plate, incubated at 37℃ for 3 days, and subsequently analyzed by spectrophotometer.
  • PDCs were treated in media with POs-Ca/Cacl2, and the number of cells in each group was assayed(p<0.05, vs. control)
  • Proliferation of DPCs was enhanced more effectively by POs-Ca than by conventional calcium.
  • Specification

    Appearance Whitepowder
    Solution Clear
    pH(1% aq) 5.5〜7.0
    Heavy metal(as Pb) ≦ 20ppm
    Arsenic((As203) ≦ 2ppm
    Loss of dryimg ≦ 6.5%
    Residue on ignition ≦ 1.9%*
    Calcium 4.5〜5.5%*
    Total aerobic microbial count ≦ 100CFU/g
    Total years and moulds count ≦ 20CFU/g
    Escherichia coil Negative
    *by dry weight

    Name

    Trade name POs-Ca
    INCI name Calcium Phosphoryl Oligosaccharides

    Other Infomation

    Cas No. 1124316-47-7
    for enquires, contact Glico Nutrition Co., LTd., Sales Division, Global Sales Group 4-6-5, Utajima, Nishiyodogawa-Osaka 555-8502 Japan
    FAX: +81 6-6477-8673
    E-MAIL: g-ingredients@glico.ccom